Objective To evaluate the application effect of labor education in practical teaching of Basic Nursing. Methods A total of 50 vocational nursing students enrolled in 2021 were treated as a control group, and their counterparts （n=48） enrolled in 2022 were regarded as an experimental group. In skills teaching of Basic Nursing, the control group received traditonal teaching method, while the experimental group received the teaching plan based on the concept of labor education, integrating knowledge, skills and literacy.At the end of the semester, the skills training test results, labor literacy and students′ evaluation of the teaching were compared between the two groups. Results In the final skills training and the overall evaluation result of skills training, the experimental group scored significantly higher than those of the control group; and except for the dimension of labor values, the scores of other dimensions and the overall score of labor literacy of the experimental group were significantly better than those of the control group （all P＜0.05）, and the students′ evaluation for the skills training in the items of autonomy, normativity, empathy ability and communication ability in the experimental group were significantly better than those of the control group （all P＜0.05）. Conclusion Application of the labor education program in the skills teaching of Basic Nursing, is conducive to enhancing students′ skill, improving their labor literacy, and cultivating their empathy ability and communication ability.