Objective To explore the characteristics of latent categories of daytime sleepiness among nurses working rotating shifts in intensive care units, and to analyze the influencing factors of the latent classes. Methods Using the convenient sampling method, 304 nurses working rotating shifts in ICU of 3A hospitals in Zhejiang province were surveyed online using the self-designed general data, the Chinese Adult Daytime Sleepiness Scale and the Work-Family Conflict Scale. Results Three distinctive categories were derived from the data:no/low sleepiness group（24.3%）, moderate sleepiness group（48.5%） and the high sleepiness group （27.2%）.The results of multinomial logistic regression analysis showed that work-family conflict, working years, nap before night shift and actual sleep time after night shift were the influencing factors for the latent categories of daytime sleepiness （P＜0.05 for all）. Conclusion There is heterogeneity in daytime sleepiness characteristics of ICU nurses working rotating shifts.Nursing managers should pay attention to nurses′ daytime sleep after night shifts and develop individualized interventions, in an effort to promote sleep health and improve daytime sleepiness in nurses.