Objective To explore the current situation of online academic emotion of nursing students and analyze its influencing factors. Methods Using the convenient sampling method, 1456 students who met the inclusion criteria were selected and investigated using the Academic Emotion Questionnaire, Learner Perceived Teacher Emotional Support Scale, Online Learning Engagement Scale and Online Self-regulated Learning Scale. Results The scores of online learning nursing students′ academic emotions are pleasure dimension (10.34±1.12), depression dimension (16.34±1.32) and boredomdimension (19.34±1.09). Learning achievement grade, whether it is a student cadre, learners′ perceived teacher′s emotional support, online learning engagement and online self-regulated learning are the influencing factors of online academic emotion of nursing students in higher vocational colleges (P<0.05 for all). Conclusion In online academic mood, nursing students have a high degree of depression. Schools can formulate personalized intervention strategies according to the influencing factors of students′ online academic emotion to ensure the quality of online teaching.