Objective Discuss the treatment and management methods of precision surgical instruments in the disinfection supply center. Methods The project management was applied to the management of precision surgical instruments, analyzing the influencing factors affecting the quality of precision surgical instruments in the disinfection supply center, forming a special management team of precision surgical instruments, constructing the project management mode, and carrying out multi-channel training to count the number of cleaning and disinfection of precision instruments and device and the damage rate or packaging failure rate before and after the management. Results Before and after the implementation of project management of precision surgical instruments, the number of precision surgical instruments was 6 149 and 6 496, respectively, the device damage rate and packaging failure rate were 0.24% and 0.08%, respectively, and the quality failure rate was 0.13% and 0.05%, respectively. Data were compared before and after implementation (P<0.05), which is statistically significant. Conclusion Project management can improve work efficiency and reduce costs cleaning quality failure rate and damage rate of precision surgical instruments, and meet the clinical supply demand.