Objective To identify the latent class of kinesiophobia characteristics in atrial fibrillation (AF) patients after radiofrequency catheter ablation (RFCA), in order to provide reference for clinical accurate intervention. Methods 475 post-RFCA patients who were followed up in the cardiology outpatient department of four tertiary hospitals in Shanghai were selected as the study subjects. They were investigated by general information questionnaire, Tampa Scale for Kinesiophobia Heart (TSK-SV Heart) and Perceived Social Support Scale (PSSS). Latent profile analysis (LPA) was used to explore the latent class of kinesiophobia, and ordinal logistic regression analysis was used to compare the characteristics of each latent class. Results The results of LPA showed that there were significant individual differences in kinesiophobia in post-RFCA patients, which could be divided into three latent profiles，the rate of low level kinesiophobia group was 29.05%, the rate of moderate level kinesiophobia group was 48.21% and the rate of high level kinesiophobia group was 22.74%. Three groups showed statistically significant differences in age, education level, occupational status, residential form, AF duration, symptom classification, and PSSS score (P<0.05 for all). Ordinal logistic regression analyses showed that elementary school and belowand symptom classification were risk factors for kinesiophobia, whereas AF duration <3 years and PSSS scorewere protective factors for kinesiophobia (P<0.05 for all). Conclusions There is significant heterogeneity in the level of kinesiophobia in AF patients after RFCA. Medical staff should carry out targeted intervention strategies to reduce the level of kinesiophobia according to the characteristics of different populations.