Objective To explorethe application effect of modified 70° lateral position in Da Vinci robotic-assisted partial nephrectomy. Methods A total of 216 patients undergoing Da Vinci robotic-assisted partial nephrectomy were divided into a conventional group (n=100) and a modified group (n=116) chronologically. During the operation, the longitudinal plane of the back of the conventional group showed 90° to the plane of the operating bed, which showed 70° in the modified group. Results In the modified group, the position placement time, operation time and renal artery blocking time were significantly shorter than those in the conventional group, the patients comfort and physicians satisfaction were significantly better, and the number of robotic arm collides with the torso was significantly less than that of the conventional group (all P<0.05); while there weren′t significant differences in intraoperative blood loss and the incidence of robotic secondary berth between the two groups (both P>0.05). Conclusion Application of the modified 70° lateral position in the Da Vinci robotic-assisted partial nephrectomy can facilitate surgical operation, improve patient safety and comfort, and it is conducive to improving nursing quality.