Objective To investigate incidence of pulmonary infection in non-acute stroke patients undergoing tracheotomy, and to analyze its influencing factors, so as to provide a reference for developing targeted preventive interventions. Methods The clinical data of 181 non-acute stroke patients undergoing tracheotomy in the rehabilitation department were retrospectively collected, the incidence of pulmonary infection was investigated, and its influencing factors were analyzed using logistic regression analysis. Results The incidence of pulmonary infection was 43.6%, and a total of 72 strains of pathogenic bacteria were detected, including 66 strains of gram-negative bacteria and 6 strains of gram-positive bacteria. The logistic regression analysis showed that, hypoproteinemia, Glasgow Coma Scale score, gender and electric standing bed treatment were the main influencing factors of pulmonary infection in non-acute stroke patients undergoing tracheotomy (all P<0.05). Conclusion The incidence of pulmonary infection in non-acute stroke patients undergoing tracheotomy is high, thus it is necessary to conduct nursing intervention according to its influencing factors, so as to prevent pulmonary infection.