Objective To explore the practice effect of a collaborative gynecological nurse-led clinic under the concept of Enhanced Recovery After Surgery (ERAS). Methods A collaborative gynecological nurse-led clinic was constructed in September 2019. Qualified specialist nurses were selected for outpatient visits, work instruction and content were formulated, and the specialist nurses assisted gynecologists to implement pre-hospital examination, pre-rehabilitation, surgical scheduling, transitional care, etc. And the nursing quality control was also covered in the clinic. Results During the 3 years after the construction of the collaborative gynecological nurse-led clinic, 19 824 patients were enrolled, in which 6 080 patients completed pre-hospital pre-rehabilitation, and 4636 patients completed transitional care. During the 4 months after the construction of the clinic, the patients′ preoperative bed waiting days, average length of hospitalization, cancellation rate and 30-day readmission rate were significantly lower than those patients enrolled in 4 months before the construction, and the satisfaction of patients and physicians with the clinic was significantly higher (all P<0.05). Conclusion Construction of the collaborative gynecological nurse-led clinic could effectively improve the efficiency of gynecology department and the quality of patients′ rehabilitation, and enhance the satisfaction of patients and physicians.