Objective To extract the best practice evidence for intraoperative prone positioning of patients under general anesthesia and analyze its clinical effect. Methods Based on the PIPOST principle, studis on intraoperative prone positioning were retrieved, and the best evidence was extracted to form an evidence-based practice plan. The incidence of pressure injury was compared between the control group (n=84) and the experimental group (n=93). Meanwhile,the knowledge scores of intraoperative prone position management of 68 nurses and their implementation rates of the seven audit indicators before and after the intervention were compared. Results The nurses′ prone position management knowledge score increased from 66.53 to 78.47 after the intervention, the overall implementation rate increased from 60.03% to 82.95%, and the incidence of pressure injury decreased from 11.90% to 3.23% (all P<0.05). Conclusion The application of the best evidence of prone position in general anesthesia patients, can standardize nursing behavior and lower the rate of pressure injury.