Objective To investigate the knowledge-attitude-practice （KAP） status and training needs of clinical nurses regarding pa-renteral nutrition in hospitalized patients, and to provide reference for clinical training. Methods A total of 2,701 clinical nurses from 33 hospitals at different levels in 16 prefecture-level cities in Hubei Province were conveniently selected for online questionnaire survey by using a self-made parenteral nutrition KAP questionnaire. Results The scoring rate of knowledge, attitude and behavior dimensions of parenteral nutrition were 53.90%, 90.45% and 85.87% respectively.And the overall scoring rate of KAP was 77.00%. Univariate analysis showed that working years and training were the influencing factors of clinical nurses′ parenteral nutrition knowledge, attitude and behavior; whether the hospital had a clear parenteral nutrition management system, whether the hospital would regularly carry out quality control of parenteral nutrition were the influencing factors of attitude and behavior (P＜0.05 for all).About 16.62% (449/2701) of the nurses thought that their knowledge was not enough to meet the needs of current clinical work;more than 80% of the nurses hoped to receive training on prevention and treatment of parenteral nutrition complications, the selection and maintenance of parenteral nutrition infusion lines. Conclusion Clinical nurses have a positive attitude to-wards the implementation of parenteral nutrition in hospitalized patients; they also have good behaviors and poor knowledge.Nur-sing managers should establish and optimize the training mechanism, strengthen the knowledge and behavior of clinical nurses, and improve nursing care for patients receiving parenteral nutrition.