Objective To construct a bedding and clothing cleaning up/decontamination protocol for thyroid cancer patients on 131I therapy, and to provide reference for clinical management of bedding and clothing used by these patients. Methods Through clinical investigation, literature study, analysis of influencing factors, semi-structured interviews and group discussions, the draft of bedding and clothing cleaning up/decontamination protocol for thyroid cancer patients on 131I therapy was constructed.Then 30 experts were selected to join 2 rounds of Delphi expert consultation, and finally the protocol was constructed. Results The response rate of the two rounds of expert consultation questionnaire was 100% （30/30）.In the second round of expert consultation, the coefficient of variation of each item was between 0.00 and 0.24, the significance scores was between 4.13 and 5.00; expert authority coefficient was 0.867, Kendall′s coefficients of concordance for first-level, second-level and third-level indicators were 0.475, 0.420 and 0.292, respectively （all P＜0.05）, and the finalized protocol included 5 first-level indicators, 20 second-level indicators and 59 third-level indicators. Conclusion The establishment process of the bedding and clothing cleaning up/decontamination protocol for thyroid cancer patients on 131I therapy is rigorous, and the protocol can provide guidance for clinical management of bedding and clothing used by thyroid cancer patients on 131I therapy.