Objective To investigate the effect of different durations of skin-to-skin contact on early breastfeeding behavior of new-borns after Cesarean section. Methods A total of 140 women with a viable singleton pregnancy scheduled for elective Cesarean section were randomly divided into 4 groups, with 35 women in each group.The control group was given routine Cesarean section care.Intervention group 1 had skin-to-skin contact with newborn for 30 minutes, intervention group 2 for 60 minutes, and intervention group 3 for 90 min.The start time of newborns′ first foraging sign, of first nipple sucking and of first breastmilk sucking, and the success rate of early breastfeeding were compared among the 4 groups. Results The start time of newborns′ foraging signs of newborns in the three intervention groups were significantly earlier than that in the control group （all P＜0.05）; the start time of nipple sucking, as well as start time of first breastmilk sucking in intervention group 2 and 3 was significantly earlier than that in the control group （P＜0.05 for all）.The success rate of early breastfeeding in intervention group 2 and 3 was significantly higher than that in the control group （P＜0.008）. Conclusion Mother-newborn skin-to-skin contact for 60 and 90 minutes is beneficial to the establishment of early breastfeeding behavior of newborns after Cesarean section.