Objective To summarize the experiences of nursing cooperation in portable wireless ultrasound-guided radial artery catheterization. Methods A total of 51 patients received portable wireless ultrasound-guided radial artery catheterization. The experiences of nursing cooperation included patient preparation, preparation of instruments and consumables, performing the Allen test, placing body position for puncture, assisting the physician to insert the catheter, providing puncture site care, and preventing complications. Results All patients successfully received radial artery cannulation, with the first attempt success rate of 86.28%. One patient required five attempts and developed hematoma at the puncture site.The rest patients had no infections, vasospasm, atherothrombosis, nerve injury and other complications. Conclusion Efficient and standardized nursing cooperation can enhance the success rate of ultrasound-guided radial artery cannulation and reduce complications.