Objective To investigate the humanistic practice ability among master of nursing specialist students, and to analyze its influencing factors, so as to provide a reference for conducting nursing education. Methods A total of 300 master of nursing specialist students were surveyed using a general data questionnaire, the Nurse Humanistic Practice Ability Scale (NHPAS) and the Nursing Professional Values Scale-R (NPVS-R). Results The participants′ NHPAS score was (100.61±13.40) points. Multiple linear regression analysis showed that, marital status, years of clinical nursing work before master education, family care and the score of NPVS-R were the influencing factors of humanistic practice ability in master of nursing specialist students (P＜0.05 for all). Conclusion The humanistic practice ability in master of nursing specialist students needs to be improved, nursing educators should develop targeted cultivation programme, strengthen students′ awareness of humanistic care, train humanistic care skills, create a humanistic care atmosphere and pay attention to the guidance of professional values, so as to enhance their humanistic practice ability.