Objective To analyze the relationship between pain-related patient-reported outcomes and kinesiophobia level in patients after video assisted thoracoscopic surgery for lung cancer. Methods A total of 212 patients undergoing video-assisted thoracoscopic surgery for lung cancer were investigated on postoperative day 1 using the Tampa Scale for Kinesiophobia-11 and the International Pain Outcomes Questionnaire. Results The incidence of kinesiophobia was 38.68%, and the median score of kinesiophobia was 25 (interquartile range 23-29). Female patients were more likely to develop kinesiophobia.Pain intensity, physical and emotional functional interference, and perceptions of care were significantly associated with kinesiophobia level (all P＜0.05). Conclusion Kinesiophobia is prevalent in patients after video-assisted thoracoscopic lung cancer resection, and poor pain-related patient-reported outcomes increase kinesiophobia level.Pain characteristics and psychological features of patients should be addressed to reduce their fear of postoperative rehabilitation exercise.