Objective To investigate medical staff′s knowledge-attitude-practice (KAP) on medical disclosure, and to analyze the influencing factors, so as to provide reference for promoting the quality of medical disclosure and protecting patients′ rights. Methods A total of 3329 medical staff from three grade A hospitals in Xinjiang were investigated using a self-designed knowledge, attitude, practice on medical disclosure questionnaire. Results The total average score of the questionnaire was (121.45±15.41), and the subscale score of knowledge, attitude and practice was (4.71±0.84),(53.98±9.49) and (62.77±8.44) respectively.Totally 2 425(72.8%) participants understood medical disclosure, 715(21.5%) were very familiar with the related management system of medical disclosure in hospital, 730(21.9%) were very satisfied with the management behavior of medical disclosure.Regression analysis showed that, age, occupational role, working department and whether had participated in medical disclosure training were the influencing factors of medical staff′s KAP score on medical disclosure (P＜0.01 for all). Conclusion Medical staff′s KAP on medial disclosure is generally good.However, most medical staff don′t know the medical disclosure management system in hospital, and are not satisfied with the management behavior.Hospital managers should pay attention to quality control of medical disclosure, and related training, supervision and feedback should be conducted for medical staff, so as to standardize their medical disclosure behavior gradually.