Objective To survey the willingness of nurses in 3A hospitals to participate in "Internet Plus nursing service", to analyze the influencing factors, and to provide reference for the smooth development of "Internet Plus nursing service" in Huzhou. Methods A questionnaire survey on willingness to participate in "Internet Plus nursing service" was conducted among 500 nurses from Huzhou 3A hospitals. The nurses were all qualified for national "Internet Plus nursing service". Results Totally，30.80% of the nurses were familiar with "Internet Plus nursing service" policy;37.00% of the nurses were willing to participate in "Internet Plus nursing service", 18.40% refused, and 44.60% held a wait-and-see attitude. Nursing services with high participation intention included nursing care of PICC/infusion port (64.95%), intravenous injection (63.97%), nursing care of indwelling drainage tube (63.48%), intravenous infusion (63.24%), subcutaneous and intramuscular injection (62.25%), and nasal feeding (61.27%). Nurses′ awareness of related policy, adequacy of spare time,and "Internet Plus nursing" experience were the main factors influencing nurses′ willingness to participate in "Internet Plus nursing service". Conclusion The overall willingness of nurses to participate in "Internet Plusnursing service" is low. Nurses who have abundant spare time, who have experience in "Internet Plus nursing service", and who are aware of related policy are more willing to participate in "Internet Plus nursing service". Measures should be taken to ramp up publicity of "Internet Plusnursing service", in a bid to orderly push forward the rollout of such service.