Objective To study the effect of non-nutritive sucking combined with facilitated tucking on pain inflicted by intramuscular injection in full-term neonates. Methods Totally, 206 full-term neonates were divided into 2 groups using the random number table. The control group （n=102） was soothed with routine comfort measures (as in patting, stroking, etc.) before receiving intramuscular injection, while the intervention group (n=104) was given non-nutritive sucking combined with facilitated tucking before intramuscular injection. Pain scores were measured, after injecting drugs, at needle withdrawal, and 3 min after needle withdra-wal, and compared between the groups. Results Pain scores measured at 3 time points in the intervention group were significantly lower than those in the control group （P<0.01 for all）. Conclusion Non-nutritional sucking combined with facilitated tucking can relieve the pain inflicted by intramuscular injection in full-term neonates.