Objective To investigate the effect of preoperative prehabilitation on functional status and nutritional status of patients with adenocarcinoma of the esophagogastric junction (AEG). Methods One hundred and sixty-eight patients with AEG were divi-ded into a control group and a prehabilitation group according to operation time, with 84 in each group. During 9 to 11 weeks of preoperative neoadjuvant chemotherapy, the control group received routine nursing care, while the prehabilitation group was subjected to preoperative prehabilitation program, including exercise prehabilitation (aerobic exercise, resistance training, and respiratory exercise) and nutritional support (ameliorating disease-related symptoms, optimizing physical fitness, and balanced diet). Results A total of 75 patients in the control group and 80 in the prehabilitation group completed the study. The 6-minute walk distance, serum albumin and prealbumin on preoperative day 1 and at postoperative week 4 in the prehabilitation group were significantly higher than those in the control group (P<0.01 for all). Intravenous infusion of albumin and pulmonary infection rate after the operation were significantly lower in the prehabilitation group compared with the control group (P<0.05, P<0.01). Conclusion Preoperative prehabilitation can improve physical function and nutritional status of patients with AEG undergoing radical ope-ration in combination with neoadjuvant chemotherapy, which is conducive to completion of the whole treatment.