Objective To investigate perception of human caring among nursing students in Colleges and Universities,so as to provide references for developing targeted intervention. Methods A total of 600 nursing students from Colleges or Universities were investigated using the self-designed Nursing Students′ Perception of Human Caring Questionnaire. Results The total score of nursing students′ perception of human caring was 4.03±0.59, with the highest dimension score of peer caring (4.17±0.67) and the lowest dimension score of environment caring(3.84±0.74). There were significant differences in the scores of human caring perception among the students by whether being the only child or not, or by educational background and college year (P<0.05, P<0.01). Conclusion Nursing students′ perception of human caring in school is high, but their perception of environment caring is relatively low. Students who are not the only child, who are undergraduate and having senior college year perceive lower human caring. So, human caring strengthening program should be conducted in nursing schools to improve caring environment and nursing students′ perception of human caring.