Objective To explore the effects of relative audio music therapy combined with premature infants oral motor intervention on feeding effect, length of hospitalization and family satisfaction of preterm infants. Methods The preterm infants in NICU were divided into a control group (n=48) and an intervention group (n=52) chronologically. During hospitalization, the control group received oral motor intervention besides routine nursing, twice a day, 5 minutes each time; while the intervention group additionally enjoyed audio and/or video recorded by family members during each oral motor intervention. Results The non-nutrition sucking system score of the intervention group on the 3rd, 6th and 9th day after the intervention were significantly higher than those of the control group, its transition time of full oral feeding was significantly shorter than that ofthe control group, the consultation times by rehabilitation department was significantly less than that of the control group, the length of hospitalization was significantly shorter than that of the control group, and family members′ satisfaction at discharge was significantly higher than that of the control group (P<0.05, P<0.01). Conclusion Relative audio music therapy combined with oral motor intervention can promote preterm infants′ oral motor function, accelerate their feeding process, and promote their rehabilitation, so as to improve family satisfaction.