Objective To describe the level and associated factors of work-related motivation among junior nurses,and to provide reference for inspiring their work-related motivation and improving nursing quality. Methods A total of 211 junior nurses were investigated by utilizing the Nurse Psychological Capital Questionnaire-Revised, Nurse Psychological Contract scale (Hospital′s Responsibility), and Work-related Motivation Questionnaire. Results The nurses scored 74.68±14.56 on work-related motivation. Multiple linear regression showed that education backgrounds, team responsibility of psychological contract, and self-efficacy, resilience as well as optimism of psychological capital were influencing factors of work-related motivation among junior nurses (P<0.05，P<0.01). Conclusion Work-related motivation of junior nurses is at a moderate level and is affected by several factors, so nursing managers should take targeted interventions to inspire work-related motivation of junior nurses.