Objective To explore the effect of combining virtual and in-person learning in lab teaching of intravenous injection technique. Methods A total of 46 students in class 1 of 2017 year were selected as the intervention class, who was subjected to lab teaching of intravenous injection technique through combination of virtual and in-person learning, and 43 students in class 2 of 2017 year were selected as the control class, who was subjected to traditional in-person lab teaching. At completion of lab teaching, the students went through theoretical knowledge assessment, skill assessment and questionnaire survey to comprehensively evaluate the teaching effect. Results The results of theoretical knowledge assessment, and skill assessment in the intervention class were significantly higher than those in the control class （P＜0.01 for both）.According to the questionnaire survey, students in the intervention class were more in favor of the teaching mode they received than the control class （P＜0.01 for all）. Conclusion Combination of virtual and in-person learning in lab teaching of intravenous injection technique could significantly improve the teaching effect.