Objective To explore clinical feasibility, safety and effectiveness of fasting chain management in children with hydronephrosis under multidisciplinary cooperation mode. Methods A total of 146 children with hydronephrosis were divided by the admission periods: 78 children admitted to hospital from April 2018 to December 2018 were assigned into the control group, who were subjected to traditional fasting management preoperatively; 68 children admitted to the hospital from January 2019 to September 2019 were assigned into the intervention group, who were subjected to fasting chain management under multidisciplinary cooperation mode . The fasting status, thirst, hunger, crying, and blood glucose level of the two groups were evaluated and compared. Results The incidence rates of thirst, hunger (except in children 3 years and older) and crying before operation, and incidence rates of thirst and hunger (except in children 3 years and older) after operation in the intervention group were lower than in the control group （P＜0.05，P＜0.01）. Conclusion Preoperative fasting chain management under multi-disciplinary cooperation mode can effectively shorten the time of fasting before operation, mitigate preoperative and post-operative thirst and hunger, reduce crying of children, and do not increase the risk of vomiting and aspiration during operation.