Objective To investigate the prevalence of urinary incontinence in female nurses and its influencing factors. Methods A total of 310 registered female nurses from a tertiary hospital in Zhengzhou, were recruited to fill out the general information questionnaire, the International Consultation on Incontinence Questionnaire Urinary Incontinence Short Form Chinese (ICI-Q-SF), and the Urinary Incontinence Quality of Life Scale (I-QOL) . Results Among the nurses, 92（29.68%） nurses suffered from urinary incontinence. The non-incontinence group enjoyed significantly higher I-QOL total scores and domain scores of avoidance and limiting behaviors, psychosocial impact, and social embarrassment, than the incontinence group （P＜0.01 for all）.The results of multivariate Logistic regression analysis showed that older age, more pregnancies, more births, vaginal delivery, majorly administrative work, current or ever feeling vaginal mass prolapse contributed to cause urinary incontinence in female nurses (OR=1.366-9.725；P＜0.05,P＜0.01). Conclusion The prevalence of urinary incontinence among female nurses is high, and the quality of life of nurses suffering from urinary incontinence is low. It is suggested that the screening of urinary incontinence and related intervention care should be rolled out for female nurses, in an effort to prevent and treat urinary incontinence.