Objective To develop a multi-terminal eye & ENT nursing skills management system and to evaluate its application effect. Methods We integrated the resources such as operating standards and processes of eye & ENT nursing skills, updated the existing nursing information system, developed multi-terminal management system (PC terminal, pad terminal, and mobile terminal), and formed three-level management mode of "Nursing department, nursing skill supervision team, and head nurse". Then it was applied in clinical nursing practice from June 2018 to May 2019 involving 261 nurses, and the effects were compared with 264 nurses receiving traditional management from June 2017 to May 2018. Results After implementation of the multi-terminal system, the qualified rate of nurses′ performance in practical nursing skills examination, success rate of competence promotion and satisfaction of nurses were significantly improved (P<0.01 for all). Conclusion The implementation of multi-terminal eye &ENT nursing skills management system can effectively enhance nurses′ theoretical and practical nursing ability, realize diverse training and examination, improve satisfaction of nurses with the use of the system, and promote management level of specialty nursing practice.