Objective To summarize the best evidence on risk screening and assessment of diabetic foot. Methods We searched the related electronic databases and professional group′s websites from inception to January 5, 2020, to collect guidelines, evidence summaries, best practice recommendation reports and systematic reviews on risk screening and assessment of diabetic foot. Then we used the such tools as Appraisal of Guidelines for Research and EvaluationⅡ (AGREE Ⅱ), Assessment of Multiple Systematic Reviews (AMSTAR), JBI evidence pre-classification and evidence grading system to evaluate the quality of literatures and identify the level of evidence. Results A total of 9 publications were enrolled, including 5guidelines, 1 evidence summary report, 1 best practice recommendation report, and 2 systematic reviews. Totally 21 pieces of best evidence were summarized from 7 aspects: obtaining a comprehensive health history, risk screening and assessment of peripheral neuropathy, risk screening and assessment of peripheral vascular disease, foot skin inspection, foot pressure measuring, examination of foot deformities and footwear, the use of risk stratification systems, frequency of risk screening and assessment. Conclusion The summary of best evidence provides an evidence-based reference for medical staff to carry out standardized risk screening and assessment of diabetic foot. The evidence should be applied reasonably according to clinical environment and patients′ characteristics.