Objective To understand how medical staff perceive tooth loss risk in older adults, how they implement preventive measures, and to provide references for tooth loss risk assessment and interventions for the population. Methods A total of 357 medical staffers from a tertiary hospital were selected by convenience sampling method. A self-made questionnaire was used to investigate medical staffers′ general information, perception of tooth loss risk in older adults，and implement of preventive measures for tooth loss. Results Totally,197 (55.18%) medical staffer had experienced elderly tooth loss incidents. Staffers′ perception score of tooth loss risk in older adults averaged (47.84±7.82) points. Staffers′ perception score varied significantly by departments, working years, age groups, educational attainments, and whether having experiencing elderly tooth loss incidents (P<0.01 for all). Clinical measures to prevent tooth loss included sutures, braces, and dental consultations. Conclusion Medical staffers′ perception score of tooth loss risk in older adults was slightly below medium level. And the effectiveness of various preventive measures needs to be further verified. Managers should develop a risk assessment tool for elderly tooth loss and make standardized preventive measures to improve risk perception level.