Objective To evaluate the effect of Child Life service model on children′s pain level and parents′ anxiety level during pediatric lumbar punctures. Methods In the non-contemporaneous control study,150 children receiving lumbar puncture were treated as the intervention group, who were subjected to Child Life service model; an earlier batch of 159 children receiving lumbar puncture were treated as the control group, who received routine nursing care. Pain level during lumbar puncture in the children and anxiety level in the parents were measured. Results The rate of successful lumbar puncture had no significant difference between the 2 groups(P>0.05). The children pain scores and parent anxiety scores in the intervention group were significantly lower than those in the control group (P<0.01 for all）. Conclusion Child Life service model could relieve children′s pain level and parents′ anxiety level during pediatric lumbar punctures.