Objective To explore the effect of multi-mode training on pain resource nurses based on information technology. Methods A total of 78 nurses were selected for on-the-job training in the first phase. In the second phase, the training course system for pain resource nurses in the hospital was constructed based on the weak points in pain knowledge level and the demand for courses among 90 nurses. The trainees were trained and certified for two years by multi-mode training methods which combined online and offline training and objective structured clinical examination(OSCE). Results The trainees scores of pain knowledge and attitude and the OSCE scores in the second phase were significantly higher than those before the training (all P<0.01); the pass rate of comprehensive evaluation and satisfaction degree of trainees in the second phase were significantly higher than those in the first phase (all P<0.01); patients′ opinion about pain education by nurses was significantly better than that before training, and the number of non drug intervention was significantly more than that before training (all P<0.01). Conclusion The multi-mode training of pain resource nurses based on information technology is helpful to improve the training effect and satisfaction of trainees, and improve the praise rate of patients for nurses′ pain education and the quality of clinical pain nursing management.