Objective To enhance comprehensive nursing skills of higher vocational nursing students and help them adapt well to cli-nical nursing. Methods By analyzing problems in previous teaching, the curriculum of Comprehensive Nursing Skill Training was reformulated based on information technology, core competence of higher vocational graduates and nursing post competence, including three modules of single skill training, comprehensive skill training and test which needed to be completed in 5 periods, and skills required in the department of internal medicine, surgery, pediatrics and intensive care were also established. The curriculum was taught in 851 students who enrolled in 2017 by the school and hospital. Results The students scored (89.18±4.84) in the examination, and 82.02%-90.01% of them were satisfied with the curriculum and teaching methods. Among 131 clinical mentors,45.04%-67.18% of them thought that the students had better performance compared with the former ones. Conclusion Reformulation of Comprehensive Nursing Skill Training Curriculum and school-hospital collaborative teaching can improve teaching effect and lead to positive feedbacks of students and teachers, thus to help students adapt well to clinical work.