Objective To investigate nurses′ knowledge of and willingness to participate in Internet Plus nursing service，and to provide reference for the introduction of Internet Plus nursing service into Zhengzhou City. Methods A total of 409 nurses from 13 hospitals in Zhengzhou were selected for questionnaires survey by means of convenient sampling. Results Surprisingly，51.10% of the nurses were unaware of Internet Plus nursing service notice issued by the National Health and Health Commission; 82.89% were supportive towards Internet Plus nursing services; 64.30% were willing to participate. The service items that nurses were most willing to offer included blood sugar monitoring (75.65%), giving health guidance (74.61%), intravenous infusion (74.35%), and venous blood collection (71.76%). The service items that nurses showed the lowest willingness to participate in included enema (38.60%), bladder irrigation (37.56%), stoma care (34.46%), sputum suctioning (32.90%), etc. Univariate analysis showed that nurses with a specialist certificate, monthly income of 3000~5999 RMB, and a high level of awareness of Internet Plus nursing services were more willing to participate in Internet Plus nursing services (P<0.05, P<0.01). Conclusion Nurses have low awareness of Internet Plus nursing service，but they show a high willingness to participate. It is suggested that relevant health departments should keep in step with national policies，increase publicity of emerging care models，encourage nurses to participate actively，improve relevant laws and regulations based on nurses′awareness and willingness to participate，and guide the practice of Internet Plus nursing services in inactivated areas.