Objective To explore the effect of applying the model of knowledge, attitude and practice (KAP) in patient suicide prevention training for nurses working in emergency department. Methods A multidisciplinary anti-suicide team was established and provide suicide risk training to 143 nurses working in emergency department. The training program lasted for 3 months. Nurses′ attitude towards patient suicide and their suicide proofing practice before and after training were compared.Results The total scores of suicide attitude and scores of 3 dimensions among the nurses after training were significantly lower than before training, while nurses′ suicide proofing practice scores were significantly higher than before (P＜0.05，P＜0.01). Conclusion Applying the KAP model in patient suicide prevention training for nurses working in emergency department can effectively improve nurses′ attitude towards suicide and suicide proofing practice.