Objective To investigate the current nursing, attitude, willingness towards multi-sites practice and suggestions among nurses in nurse-led clinics from tertiary hospitals, so as to provide reference for conducting nurses′ multi-sites practice. Methods A total of 168 nurses in nurse-led clinics from tertiary hospitals in Suzhou were investigated using a self-designed nurses′ attitude and willingness towards multi-sites practice. Results Totally 45.8% nurses in nurse-led clinics had a certificate for specialized nursing issued by the city or above, 86.3% nurses practiced independently; 81.0% nurses were in favor of multi-sites practice, and 89.3% were willing to try. As for the forms of multi-sites practice, more than 60% nurses preferred to do part-time work in lower-level medical institutions or aged care institutions, and spend 4-8 hours every week in multi-sites practice. More than 90% nurses thought the benefit of multi-sites practice was allowing patients to enjoy better health care services nearby, while the difficulty was lacking of sound relevant laws and regulations as well as the practitioner safeguard system. Conclusion The nurses in nurse-led cli-nics from tertiary hospitals have rich experience in clinical nursing, and they have positive attitude and high willingness towards multi-sites practice, thus they obtain an advantage in this practice. Currently, their main concern is the safety of multi-sites practice, they hope the government and health management departments issue relevant policies and regulations as soon as possible to ensure the safety of nurses on multi-sites practice.