Objective To construct a venous vascular grading and nurse competence matching system in pediatric outpatient department, so as to match the best nurse for children automatically and precisely, and improve the success rate of venipuncture. Methods We established the pediatric vascular grading and nurse puncture competence criteria, and constructed a venous vascular grading and nurse competence matching system, including vascular grading data module, nurse puncture competence data module and infusion room decision support module, then the data were integrated and transferred via pediatric outpatient transfusion nursing management information system and HIS, and the system recommended a matching puncture nurse for each child. The number of punctures per day per nurse, the first puncture success rate, the complaint reports of punctures and the satisfaction of nurses and children′s parents before and after using the system were calculated and compared. Results After using the system, the daily average number of punctures per nurse and the first puncture success rate were significantly higher than those before using the system (P<0.05, P<0.01), parents′ satisfaction towards nursing work and nurses′ job satisfaction were improved. Conclusion Construction and application of the venous vascular grading and nurse competence matching system in pediatric outpatient department could increase the first puncture success rate, enhance the management quality of peripheral vein, and finally improve satisfaction of nurses and parents.