Objective To explore the application effect of video-based case tracking method in the quality control of treatment process for Acute Coronary Syndrome (ACS). Methods Totally 234 patients with ACS were divided into a control group (n=106) and anintervention group (n=128) chronologically. Conventional quality control method was used for the control group, while video-based case tracking method was utilized in the quality control of the intervention group, including regular case review, on-site inspection, regular assessment of medical staff and interview of clients and their family members. Results Time elapse of triage, first electrocardiogram, troponin acquisition, dual antagonists use, activation of conduit room, the door to balloon and the initial treatment of ACS patients in the intervention group were significantly shorter than those in the control group, while patients′score toward process nursing was significantly higher than that of the control group (P<0.01 for all). Conclusion Application of video-based case tracking method in quality control of the treatment process can effectively improve the timeliness of treatment for patients with ACS, and it is significant to standardize the process management of chest pain center.