Objective To explore a formative assessment system suitable for training of nursing talents through higher education. Methods Totally, 186 undergraduate nursing students admitted to Liaoning University of Traditional Chinese Medicine in 2015 were divided into 2 classes for the course of obstetric and gynecologic nursing care.Then the control classes （n=94） were evaluated with class average achievement gains and final exam performance, while the evaluation of the intervention class （n=92） consisted of five other parts besides final exam performance: class signing, classroom testing, stage testing, experimental class results, and results of clinical probation. Results There were significant differences in the results of final written exam, comprehensive evaluation results and teaching results between the two classes （P＜0.01 for all）. Conclusion The formative evaluation system based on the Dui Fen Yi Network Teaching Platform could timely and effectively evaluate students′ learning process, consolidate students′ professional knowledge, improve their self-directed learning ability, and enhance the learning effect.