Objective To evaluate the effect of various locking solutions for hemodialysis catheters in adults patients. Methods PubMed, Cochrane Library, Embase, China National Knowledge Infrastructure (CNKI), Wanfang Data, and VIP database were searched to collect randomized controlled trials （RCTs） on usage of different locking solutions to lock the hemodialysis catheters in adult patients, with the publication time being limited to database inception to September,2018. The Cochrane 5.1.0 systematic review manual was used for quality evaluation, the Stata 12.0 software was used to draw the network diagram, and the WinBUGS was used for data analysis. Results A total of 15 publications were included, reporting 8 kinds of locking solutions, with a total sample size of 2 159 cases. The results of network meta analysis showed no statistically significant differences in 3 outcome mea-sures (catheter-related infections, infection at the catheter interface, and death).However, from the probability ranking table, the optimal locking solutions were antibiotics, citrate and ethanol, which were beneficial to the reduction in catheter-related infections, infection at the catheter interface and the occurrence of death. Conclusion Through a combination of the results of network meta analysis and the probability ranking table, given that long-term use of antibiotics can lead to bacterial resistance, ethanol or citrate can be recommended as a sealing solution for hemodialysis patients.