Objective To enhance the efficiency of the operating room. Methods Through multisectoral collaboration, these measures were taken in the operating room to enhance the efficiency: perfecting environment and equipment, optimizing work duty and process, ensuring timely start of the first operation, using technologies, improving bonusmalus system, and establishing problem solving response mechanism. Average duration between two operations, rates of timely start of the first operation and delayed operation, as well as patient and surgeon satisfaction were compared before (January to December 2016) and after the intervention (April 2017 to March 2018). Results The average duration between two operations was shortened, the rate of timely start of the first operation was increased, and the rate of delayed operation was decreased after the intervention (P<0.01 for all). Satisfaction of patients and surgeons with nursing work were enhanced (P<0.05,P<0.01). Conclusion Multi-sectoral collaboration in the operating room can improve work efficiency, and enhance satisfaction of patients and surgeons.