Objective To investigate the situation of sterile supply service in 33 hospitals in Banan District, Chongqing, and to explore the feasibility of building a regional central sterile supply center (CSSC). Methods Chief figures in charge of sterile supply service in 33 primary hospitals in Banan District, Chongqing, were surveyed in terms of current allocation of sterile supply resources within the hospital they worked with, and their support level of a regional CSSC.The cross-sectional survey results were compared with domestic industry standards of sterile supply services, and the differences between sterile supply services in the 33 hospitals and domestic industry standards were identified. Results Of the 33 hospitals, 22 (7 2A hospitals, 14 1A hospitals, and 1 hospital of private and other nature) had sterile supply rooms;12 hospitals had never trained the sterile supply service staff.Among the 22 hospitals which had sterile supply rooms, the qualified rate of "three zones"was 59.09%, the qualified rate of "decontamination zones" was 36.36%(8 hospitals), the qualified rate of"packaging sterilization zones" was 45.45%(15 hospitals);68.18%(15 hospitals) were qualified with the lowest staffing.Only 4 hospital received the certificate of Standard Management of Central Sterile Supply Department (CSSD).Chief figures from 27 hospitals(81.82%) supported building of a regional resource-sharing CSSC. Conclusion There are serious defects in the layout, facilities, staffing,etc.,of the 33 hospitals in Banan district, Chongqing.Therefore, by relying on the existing standardized CSSDs, and innovating the working mode, a regional resource-sharing CSSC, which can achieve the integration and sharing of resources and reduce repeated investment in disinfection facilities, can be built to make possible centralized management of recycled articles, and improve the quality of disinfection and sterilization services in primary hospitals.