Objective To investigate the effect of intestinal rehabilitation training on chemotherapy-related constipation in patients with lung cancer. Methods A total of 172 lung cancer patients on chemotherapy who were given 5-HT3 receptor antagonists as an-tiemetic and developing constipation were randomly divided into 2 groups, with 86 cases in each group. Routine nursing care was given to all the patients. Besides that, the control group was instructed to take Duphalac orally, while the intervention group was subjected to daily intestinal rehabilitation training.The intervention lasted 7 days.Changes in constipation, before and after anxiety levels and constipation-related quality of life in the 2 groups were compared. Results The time to first defecation, the time to pass normal shaped stool, in the intervention group were significant shorter than the controls; the former had more frequent defecation within 3 days, and within the 3-7 days range, than the latter （P＜0.05,P＜0.01）.On the 7th day, anxiety levels and constipation-related quality of life in the intervention group were significant lower than the controls（P＜0.05 for both）. Conclusion Intestinal rehabilitation training can significantly improve the condition of chemotherapy-related constipation and bad mood in patients with lung can-cer, thus enhance their quality of life.