Objective To investigate the current status and influencing factors of moral injury symptoms among new nurses, and to provide a reference basis for the development of relevant interventions.Methods From February to April 2025, a convenient sampling method was used to select new nurses from 6 tertiary hospitals in Tianjin as research subjects.The general information questionnaires, the Moral Injury Symptom Scales, and the Trait Meta-Mood Scales were used for the survey.Univariate analysis, correlation analysis, and multiple linear regression analysis were conducted to analyze the influencing factors of moral injury symptoms among new nurses.Results A total of 345 new nurses completed the survey.The total score of moral injury symptoms was (63.86±16.66) points, and 67.25% of new nurses had moral injury symptoms.The results of multiple linear regression analysis indicated that educational level, whether being an only child, workplace adaptability, family concern, and trait meta-mood were the influencing factors of moral injury symptoms among new nurses (all P＜0.05).Conclusion New nurses exhibit relatively obvious symptoms of moral injury and a compromised state of well-being.Nursing managers can take corresponding intervention measures based on the influencing factors to help new nurses improve workplace adaptability, encourage family support, maintain the physical and mental health of new nurses, so as to prevent and treat moral injury among new nurses, and optimize the construction of the nursing team.