Objective To investigate the current status of knowledge, attitude, and practice (KAP) towards oral nutrition supplementation (ONS) in clinical nurses, and to analyze its influencing factors, in order to provide a reference for conducting relevant training in clinic.Methods A convenience sampling method was used to select clinical nurses from 19 hospitals in Hubei Pro-vince, then they were investigated by using a general information questionnaire and a self-made questionnaire on nurses′ know-ledge, attitude, and practice towards ONS.Results A total of 1 628 nurses were ultimately included in the study, with a total score of KAP at （77.15±13.00） points, knowledge dimension at （8.76±2.61） point, attitude dimension at （30.70±6.45） points, and practice dimension at （37.70±8.29） points.The results of multiple linear regression analysis showed that, department, working years, whether the hospital/department having ONS management process and the frequency of participation in training were the influencing factors of knowledge, attitude and practice of ONS in clinical nurses （all P＜0.05）.Conclusion The KAP towards ONS in clinical nurses is generally at a medium and above level, while their knowledge is insufficient, their attitude is positive, and their practice needs improvement.Nursing managers should standardize the use and management process of ONS, strengthen related knowledge training, and further improve the KAP level towards ONS in clinical nurses.