Objective To explore the current status and influencing factors of humanistic caring behaviors among nursing assistants in hospitals, so as to provide a reference for managers conducting targeted training to improve nursing assistants′ human-centered care quality.Methods A general information questionnaire and the Caring Behaviors Inventory (CBI) were utilized to investigate 413 nursing assistants from four tertiary hospitals in Zhejiang Province.Results The participants′ overall score of CBI was （94.62±14.28） points, and the subscales of respect and support was （4.91±0.13） points, knowledge and skills was （4.57±0.20） points.Multiple linear regression analysis demonstrated that, marital status, years of working experience, whether standardized humanistic care training had been received, and family harmony were major influencing factors of humanistic caring behaviors in nursing assistants （all P＜0.05）.Conclusion The humanistic caring behaviors of nursing assistants in hospitals is at a moderate le-vel.Hospital administrators should raise entry-level qualifications for nursing assistants, establish professional standards, and strengthen their training in humanistic care competencies to further improve their humanistic caring behaviors.