Objective To explore the application effects of case-based teaching according to STEM education concept and Tanner model in Nursing Comprehensive Training.Methods Nursing undergraduates enrolled in 2021 were selected as participants.Two classes were randomly assigned via lottery as an observation group and a control group, with 102 students in each group.The control group received conventional case-based teaching in Nursing Comprehensive Training, while a teaching resource repository was developed based on the STEM education concept for the observation group, which mainly consisting of an operational skills project library and a comprehensive teaching case database, then structured case-based teaching according to the Tanner model was conducted across four phases:noticing, interpreting, responding, and reflecting.Results After the teaching, the course examination score, the critical thinking ability score, and humanistic caring ability score in the observation group were significantly higher than those in the control group （all P＜0.05）.Additionally, 67.6%-90.2% students in the observation group expressed approval for the teaching effect.Conclusion Practice of case-based teaching according to STEM education concept and Tanner model in Nursing Comprehensive Training, is conducive to enhancing students′ course examination score, improving their critical thinking ability and humanistic care ability, and students approval the teaching.