Objective To explore the application effect of curriculum humanities teaching model based on humanistic quality training in clinical practice teaching of nursing undergraduates.Methods Undergraduate nursing students were divided into two groups chronologically, fifty-seven undergraduate nursing students who underwent rotational internships from July 2022 to April 2023 were assigned to a control group, and 57 undergraduate nursing students who underwent rotational internships from July 2023 to April 2024 were assigned to an experimental group.The control group were taught using the routine clinical practice teaching mo-del, while the experimental group additionally adopted the curriculum humanities teaching model.The examination scores, professional identity, humanistic care ability and teaching evaluation of the two groups were assessed and compared.Results Totally 56 nursing interns in the experimental group and 54 nursing interns in the control group completed the study.The practical examination score, the total scores of career identity and humanistic care ability of the experimental group were significantly higher than those of the control group （all P＜0.05).The evaluation scores of teaching organization and arrangement, teaching content, tea-ching teachers, learning opportunities and overall harvest in the experimental group were significantly higher than those of the control group （all P＜0.05).Conclusion In the clinical practice teaching, conducting the curriculum humanities teaching model based on humanistic quality training can improve nursing interns′ clinical practice ability and professional identity, and effectively promote their cultivation of humanistic quality.