Objective To probe into the knowledge of, attitude towards, and practice of hierarchical medical service in palliative care among healthcare providers working in non-oncology wards, and to provide a reference for administrators to establish and implement strategies boosting the quality of palliative care referral service. Methods Totally, 613 healthcare providers working in non-oncology wards were surveyed by using the Knowledge-Attitude-Practice (KAP) Questionnaire of Hierarchical Medical Service in Palliative Care. Results The healthcare providers scored （90.80±16.73） points in attitudes, and （42.29±12.06） points in practice, respectively.Multiple linear regression analysis showed that, whether there was a referral policy for terminally ill patients in the affiliation the healthcare providers worked with, and gender influenced their attitude and practice scores （all P＜0.05）. Conclusion The healthcare providers working in non-oncology wards had various levels of knowledge regarding hierarchical medical service in palliative care, and their attitude and practice were at medium level.It is suggested that administrators pay attention to the trai-ning of non-oncology medical staff regarding hierarchical medical service in palliative care, so as to promote the development of palliative care.