Objective To evaluate the effect of implementing an evidence-based enteral nutrition (EN) practice program developed based on the RE-AIM framework for infants with congenital heart disease (CHD). Methods In this study, CHD infants admitted to the cardiovascular center of our hospital were selected as the target subjects.From January to June 2019, 98 CHD infants were enrolled in to the control group, and medical staff carried out EN practice for them according to routine management and work mode.From September 2019 to February 2020, 81 CHD infants were enrolled in to the intervention group.Then medical staff followed the principles of implementation strategies in the 2013 WHO Implementation Research Guidelines to develop strategies (including communication strategies, implementation process strategies, integration strategies, capacity building strategies) aiming to promote the implementation of interventions, and applied those strategies in infants′ EN management.Based on the RE-AIM framework, the implementation process of the nutrition program was evaluated from accessibility, adoption and implementation aspects, while implementation effect was evaluated from effectiveness and sustainability. Results The accessibility, adoption, and implementation of the nutrition program rated by medical staff were 97.7%, 92.3%, and 100%. Knowledge questionnaire scores for infant CHD enteral nutrition among medical staff before, during, and after implementation were (18.20±6.44) points, (29.95±4.18) points, (31.55±3.89) points, respectively, and the difference was statistically significant (F=265.444,P＜0.001). Conclusion The implementation strategies adopted during the rollout of the evidence-based EN practice program for CHD infants can promote the accessibility, adoption and implementation of the program by medical staff, and effectively improve their knowledge level and implementation behaviors.