Objective To understand cancer patients′ knowledge of, attitude towards, and selection preference of life and death education,and to provide reference for the development of targeted life and death education intervention program for cancer patients. Methods A convenience sampling method was used to select 300 cancer patients to fill out a self-made questionnaire on life and death education regarding cancer patients′ knowledge of, attitude towards, and selection preference of life and death education. Results The patients scored a median of 3.00 （IQR:3.00,3.00） points in knowledge, 20.00 （16.00,21.00） points in attitude, and 46.00 （40.00,50.75） points in selection preference, respectively.Education levels, tumor sites, and whether or not having a caregiver were influencing factors of cancer patients′ knowledge of, attitude towards, and selection preference of life and death education （all P＜0.05）. Conclusion Cancer patients′ knowledge of life and death education needs to be improved, and medical staff should focus on and integrate life and death education in the care of clinical cancer care.