Objective To explore the application effect of workshop combined with video teaching mode on improving new nurses′ skills in intraosseous infusion. Methods A control group consisted of 36 new nurses in July 2021. 36 new nurses were recruited to the observation group in July 2022. The control group received routine training methods for intraosseous infusion training. The observation group adopted a workshop combined with video teaching mode for intraosseous infusion skill training. After 2 weeks of training, the two groups of nurses were assessed for their theoretical knowledge and operational skills. A questionnaire survey was conducted to investigate the critical thinking ability of new nurses and their satisfaction with teaching quality. Results The theoretical assessment scores, operational assessment scores, and critical thinking ability scores of the observation group were significantly higher than those of the control group (all P<0.05). The observation group nurses had a higher satisfaction evaluation of teaching quality. Conclusion The application of workshop training combined with video teaching mode in the training of intraosseous infusion skills for new nurses can promote their mastering theoretical knowledge and operational skills, and can also enhance their critical thinking ability and satisfaction with teaching quality.